Almeida, Marilia O. 1 ; Lopes, Adriana A. 2; Roberto, Patricia G. 2; Bertoni, Bianca W. 2; Pupo, Monica T. 1
The fermentation of the endophyte Fusarium oxysporum SS46 and Fusarium oxysporum ATCC MYA 4623 with epigenetic modulator hydralazine in Czapek medium led to the isolation of 3-methyl-1,2,4-triazolo [3,4-alpha] phthalazine (1). The mechanism of biotransformation involves reaction with acetyl-CoA and further cyclization, and was unveiled using C-13-precursor. Additionally, AFLP technique for genomic DNA and differential gene expression (cDNA) analyses were applied for comparative profiling of the fungus under and without treatment with hydralazine. There was no change in the DNA profile of hydralazine-treated and non-treated cultures; however, cDNA-AFLP analyses showed a differential gene expression in the cultures after treatment of the fungi with hydralazine.
1 School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Ribeirão Preto, Brazil
2 Unidade de Biotecnologia, Universidade de Ribeirão Preto, Ribeirão Preto, Brazil
Link para o artigo completo: https://www.sciencedirect.com/science/article/pii/S1874390017307516