Authors:
Pilon, Alan C. 1,2,3 ; Selegato, Denise M.2,4 ; Fernandes, Richard P. 2 ; Bueno, Paula C. P.1,5 ; Pinho, Danielle R. 1 ; Carnevale Neto, Fausto 5 ; Freire, Rafael T. 7 ; Castro-Gamboa, Ian 2 ; Bolzani , Vanderlan S.2 ; Norberto P. Lopes 1
Abstract:
Metabolomics has played a central role in various areas of plant sciences, offering new perspectives for the advancement of agriculture, drug discovery, chemical ecology and taxonomy. Plant metabolomics (identification and quantification) aims to understand the relationship between biological systems and genetic, pathological and or environmental stimuli in terms of differential expression of the metabolism. Owing to the unique challenges, such studies require multidisciplinary skills involving biology, chemistry, statistics, and computer science for the extraction and complete understanding of information. In this sense, this review summarizes the main procedures that involve the steps of plant metabolomic study (design of experiments, sample preparation, analytical methods and data analysis), providing a comprehensive overview, showing the main challenges and limitations and possible solutions for the different approaches used.
1 Núcleo de Apoio à Pesquisa em Produtos Naturais e Sintéticos, Faculdade de Ciências Farmacêuticas de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto – SP, Brasil
2 Núcleo de Bioensaios, Biossíntese e Ecofisiologia de Produtos Naturais, Universidade Estadual Paulista, Araraquara – SP, Brasil
3 Department of Life Sciences, Imperial College London, Silwood Park, Ascot, Reino Unido
4 Center of Magnetic Resonance, University of Florence, Florença, Itália
5 Max-Planck Institute of Plant Molecular Physiology, Potsdam-Golm, Alemanha
6 Northwest Metabolomics Research Center, Department of Anesthesiology and Pain Medicine, University of Washington, Seattle, Estados Unidos
7 Signal and Information Processing for Sensing Systems, Institute for Bioengineering of Catalonia, Barcelona, Espanha
Link to article: https://www.scielo.br/scielo.php?pid=S0100-40422020000300329&script=sci_arttext
