Authors:
Graminha, Angelica E. [1, 2] ; Honorato, Joao [1] ; Correa, Rodrigo S. [3] ; Cominetti, Marcia R. [2] ; Menezes, Antonio C. S. [4] ; Batista, Alzir A. [1]
Astract:
This work describes the synthesis of three new ruthenium(II) complexes with gallic acid and derivatives of the general formula {[}Ru(L)(dppb)(bipy)]PF6, where L = gallate (GAC), benzoate (BAC), and esterified-gallate (EGA), bipy = 2,2 `-bipyridine and dppb = 1,4-bis(diphenylphosphino)butane. The complexes were characterized by elemental analysis, molar conductivity, NMR, cyclic voltammetry, UV-vis and IR spectroscopy, and two of them by X-ray crystallography. Cell viability assays show promising results, indicating higher cytotoxicity of the complexes in MDA-MB-231 cells, a triple-negative breast cancer (TNBC) cell line, compared with the hormone-dependent MCF-7 cell line. Studies in vitro with the MDA-MB-231 cell line showed that only Ru(BAC) and Ru(GAC) interacted with BSA. Besides that, the Ru(GAC) complex, which has a polyphenolic acid, interacted in an apo-Tf structure and function dependent manner and it was able to inhibit the formation of reactive oxygen species. Ru(GAC) was able to cause damage to the cellular cytoskeleton leading to inhibition of some cellular processes of TNBC cells, such as invasion, migration, and adhesion.
1 Departamento de Química, Universidade Federal de São Carlos – UFSCar, Rodovia Washington Luís Km 235, CP 676, 13561-901, São Carlos, SP, Brazil
2 Departamento de Gerontologia, Universidade Federal de São Carlos – UFSCar, Rodovia Washington Luís Km 235, CP 676, 13561-901, São Carlos, SP, Brazil
3 Departamento de Química, Universidade Federal de Ouro Preto (UFOP), CP: 35.400-000, Ouro Preto MG, Brazil
4 Centro de Ciências Exatas e de Tecnologia, Universidade Estadual de Goiás – UEG, BR 153, CP 459, 75132-903, Anápolis, GO, Brazil
Link to article: https://pubs.rsc.org/en/content/articlelanding/2021/DT/D0DT03490H#!divAbstract
