Molecular Cloning and Biochemical Characterization of Iron Superoxide Dismutase from Leishmania braziliensis


Brito, Camila C. B. 1 ; Maluf, Fernando V.  2 ; de Lima, Gustavo M. A. 2,4 ; Guido, Rafael V. C. 2 ; Castilho, Marcelo S. 1,3


Leishmaniasis is one of the most important neglected tropical diseases, with a broad spectrum of clinical manifestations. Among the clinical manifestations of the disease, cutaneous leishmaniasis, caused by species of Leishmania braziliensis, presents wide distribution in Brazil. In this work, we performed the cloning, expression, and purification of the enzyme superoxide dismutase of Leishmania braziliensis (LbSOD-B2) considered a promising target for the search of new compounds against leishmaniasis. In vitro assays based on pyrogallol oxidation showed that LbSOD-B2 is most active around pH 8 and hydrogen peroxide is a LbSOD-B2 inhibitor at low millimolar range (IC50 = 1 mM).

1  Programa de pós-graduação em Biotecnologia, Universidade Estadual de Feira de Santana, Feira de Santana, Brazil

2  Sao Carlos Institute of PhysicsUniversity of Sao Paulo, São Carlos, Brazil

3  Faculdade de Farmácia, Universidade Federal da Bahia, Salvador, Brazil

4  MAX IV Laboratory  Lund University, Lund, Sweden

LInk para o artigo completo: https://link.springer.com/article/10.1007/s12033-018-0095-2