Redox Capacitive Assaying of C-Reactive Protein at a Peptide Supported Aptamer Interface


Piccoli, Julia 1 ; Hein, Robert 2 ; El-Sagheer, Afaf H. 2, 3 ; Brown, Tom 2 ; Cilli, Eduardo M. 1 ; Bueno, Paulo R. 1 ; Davis, Jason J. 2


Electrochemical immunosensors offer much in the potential translation of a lab based sensing capability to a useful “real world” platform. In previous work we have introduced an impedance-derived electrochemical capacitance spectroscopic analysis as supportive of a reagentless means of reporting on analyte target capture at suitably prepared mixed-component redox-active, antibody-modified interfaces. Herein we directly integrate receptive aptamers into a redox charging peptide support in enabling a label-free low picomolar analytical assay for C-reactive protein with a sensitivity that significantly exceeds that attainable with an analogous antibody interface.

1  Institute of Chemistry, São Paulo State University (UNESP), Araraquara, São Paulo, Brazil

2  Department of Chemistry, University of Oxford, South Parks Road, Oxford OX1 3QZ, U.K.

3  Chemistry Branch, Department of Science and Mathematics, Faculty of Petroleum and Mining Engineering, Suez University, Suez 43721, Egypt

Link para o artigo completo:   https://pubs.acs.org/doi/10.1021/acs.analchem.7b05374